Ceruloplasmin is is the major copper-carrying protein in the blood, and in addition plays a role in iron metabolism.
It was first described in 1948.
It is an enzyme (EC 1.16.3.1) synthesized in the liver containing 6 atoms of copper in its structure.
Ceruloplasmin carries 90% of the copper in plasma.
The other 10% is carried by albumin, albumin may be confused at times to have a greater importance as a copper carrier because it binds copper less tightly than ceruloplasmin.
Ceruloplasmin exhibits a copper-dependent oxidase activity, which is associated with possible oxidation of Fe2 (ferrous iron) into Fe3 (ferric iron), therefore assisting in its transport in the plasma in association with transferrin, which can only carry iron in the ferric state.
The molecular weight of human ceruloplasmin is reported to be 151kD.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4.
Specificity：Recognizes mouse CP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Tyr729-Gly106 of mouse CP, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
