Tubulin is a major cytoskeleton component that has five distinct forms, designated alpha, beta, gamma, delta and epsilon Tubulin.
alpha and beta tubulins form heterodimers which multimerize to form a microtubule filament.
There are 5 Beta Tubulin isoforms (B1, B2, B3, B4A and B4B) that are expressed in mammalian tissues.
B1 and B4 are present throughout the cytosol, B2 is present in the nuclei and nucleoplasm, and B3 is a neuron-specific cytoskeletal protein.
gamma Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome.
Both delta Tubulin and epsilon Tubulin are associated with the centrosome.
delta Tubulin is a homolog of the Chlamydomonas deltaTubulin Uni3 and is found in association with the centrioles, whereas epsilon Tubulin localizes to the pericentriolar material.
Epsilon Tubulin exhibits a cell cycle-specific pattern of localization; first associating with only the older of the centrosomes in a newly duplicated pair, and later associating with both centrosomes.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human TUBA3C/TUBA3D/TUBA3E protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa400-450 of human TUBA3C.
