Tuberous sclerosis (TSC) is a human genetic disorder characterized by mental retardation and the widespread development of benign and infrequently malignant tumors in a variety of tissues.
Two different genetic loci have been linked to TSC; one of these loci, the tuberous sclerosis-2 gene (TSC2), encodes a protein 1784 amino acids in length, called tuberin.
Tuberin exhibits a region of limited homology to the catalytic domain of Rap1 GAP.
Subcellular fractionation studies have shown tuberin to be predominantly localized in membrane fractions.
Tuberin is capable of stimulating the intrinsic GTPase activity of Rap 1A, but not Rap 2, H-Ras, Rac or Rho.
TSC2 maps to human chromosome 16 and is associated with several intragenic mutations in affected patients.
The mouse homolog of the tuberin gene maps to chromosome 17.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human TSC2 protein only when phosphorylated at Ser939. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human TSC2 around the phosphorylation site of Ser939.
