Thymidine Kinase (TK1) is a highly conserved phosphotransferase that is present in most living cells.
Thymidine Kinase catalyzes the phosphorylation reaction: deoxythymidine + ATP = deoxythymidine 5'-phosphate + ADP; it is thus involved in the reaction chain to introduce deoxythymidine into the DNA.
Thymidine kinase is required for the action of many antiviral drugs, such as azidothymidine (AZT), and is is also used to select hybridoma cell lines in the production of monoclonal antibodies.
Thymidine Kinase has many clinical applications as it is only present in anticipation of cell division.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human Thymidine Kinase protein only when phosphorylated at Ser13. Species Crossreactivity: human
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from human TK around the phosphorylation site of S13.
