TAF1L (transcription initiation factor TFIID 210kD subunit) is a 1826aa protein that serves as a functional substitute for TAF II p250 in spermatogenic cells.
In somatic cells, TAF II p250, which is located on the X chromosome, is a general transcription factor which initiates preinitiation complex assembly through direct interaction with the TATA promoter element.
However, during male meiosis, sex chromosomes are transcriptionally silenced, inactivating the transcriptional activity of TAF II p250.
With an autosomal location, TAF1L functionally replaces TAF II p250 in testes.
It is documented that the gene encoding human TAF1L arose by retroposition of TAF II p250 mRNA during primate evolution after species divergence.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human TAF1 protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human TAF1.
