Na+/H+ exchangers 1-8, also designated Na+/H+ antiporters or NHE-1-8, are integral membrane proteins expressed in most mammalian tissues where they regulate intracellular pH and cell volume.
NHEs mediate the transport of hydrogen (H+) ions out of cells in exchange for extracellular sodium (Na+) ions.
While NHE-1 is ubiquitously expressed, NHE isoforms 2-8 have distinct tissue and cell type dependent expression and inhibitory characteristics.
NHE-8 is a 575aa protein that localizes apically in intestinal epithelial cells.
Expression of NHE-8 is higher in young mammals than adults.
NHE-8 gene and protein expression are highly regulated during ontogeny; this protein may play an important role in intestinal Na+ absorption during early mammalian life.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human SLC9A8 protein.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa450-500 of human SLC9A8.
