84-5134-53　［取扱停止］SCRIB 50ul　506037

特徴

• Drosophila melanogaster genes, which are categorized based on the type of protein for which they encode, represent six major classifications, including intracellular signaling proteins, transmembrane proteins, RNA binding proteins, secreted factors, transcription regulators (basic helix-loop-helix, homeodomain containing, zinc finger containing and chromatin associated) and other functional proteins.
• Morphogenesis and cell differentiation in Drosophila requires accurate control of cell division.
• Discs large (DLG), Scribble (Scrib) and Lethal giant larvae (LGL) tumor suppressor proteins regulate multiple aspects of neuroblast asymmetric cell division.
• DLG/Scrib/LGL proteins show apical cortical enrichment at prophase/metaphase and have a uniform cortical distribution.
• Mutations in the genes encoding multi-PDZ (PSD-95, discs-large and ZO-1) and the leucine-rich-repeat protein Scrib cause aberrant cell shapes and the loss of monolayer organization of embryonic epithelia.
• The human homolog, hScrib, is intracellularly localized to the vertebrate tight junction, which functions to correctly place adherens junctions.
• The PDZ domains of Scrib are predicted to bind to the consensus S/TXV at the C-terminus of proteins.
• PDZ domain proteins have been implicated at several different sites of the protein trafficking pathway, suggesting that Scrib is required for the localization of several epithelial determinants.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.