84-5134-48　［取扱停止］RXRA 100ul　506033

特徴

• The human retinoid X receptors (RXRs) are encoded by three distinct genes (RXR alpha, RXR beta, and RXR gamma) and bind selectively and with high affinity to the vitamin A derivative, 9-cis-retinoic acid.
• RXRs are type-II nuclear hormone receptors that are largely localized to the nuclear compartment independent of ligand binding.
• Nuclear RXRs form heterodimers with nuclear hormone receptor subfamily 1 proteins, including thyroid hormone receptor, retinoic acid receptors, vitamin D receptor, peroxisome proliferator-activated receptors, liver X receptors, and farnesoid X receptor.
• Since RXRs heterodimerize with multiple nuclear hormone receptors, they play a central role in transcriptional control of numerous hormonal signaling pathways by binding to cis-acting response elements in the promoter/enhancer region of target genes.
• Retinoid X receptor alpha (RXR alpha) is the founding RXR family member and is predominantly expressed in liver, kidney, epidermis, intestine and a variety of tissues.
• Knockout of the murine rxralpha gene results in embryonic lethality tentatively due to myocardial hypoplasia, which demonstrates the importance of RXR alpha to retinoid signaling in vivo.
• Biochemical evidence suggests that RXR alpha transcriptional activity is post-translationally regulated through the Ras-Raf-MAPK signaling cascade.
• MAPK-dependent phosphorylation of RXR alpha directly abrogates the ability of RXR alpha to associate with nuclear receptor coactivators.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.