RNA polymerase II (Pol II) is a multi-subunit complex responsible for catalyzing the transcription of DNA into RNA.
POLR3B (polymerase (RNA) III (DNA directed) polypeptide B), also known as C128 or RPC2, is a 1133aa protein that localizes to the nucleus and belongs to the RNA polymerase beta chain family.
Existing as the second largest component of the multi-protein RNA polymerase III (Pol III) complex, POLR3B functions as a DNA-dependent RNA polymerase that specifically catalyzes the transcription of DNA into RNA using ribonucleoside triphosphates as substrates.
The gene encoding POLR3B maps to human chromosome 12, which encodes over 1100 genes and comprises approximately 4.5% of the human genome.
Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and Trisomy 12p, which causes facial developmental defects and seizure disorders.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human POLR3B protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa330-380 of human POLR3B.
