DNA polymerase activity is essential for replication, repair, recombination and mutagenesis.
DNA polymerases can often bypass DNA lesions that block DNA replication, thereby allowing the replication of damaged DNA.
One such DNA polymerase is the distributive enzyme DNA pol ι (pol iota), which is encoded by the POLI gene.
POLI is located on human chromosome 18q21.2, a region often implicated in the etiology of many human cancers.
At thymine templates, DNA Pol ι is highly error-prone when replicating undamaged DNA in that it favors the misincorporation of guanine over the correct nucleotide, adenosine.
DNA Pol ι also promotes the replication of damaged DNA by misincorporating deoxynucleotides opposite DNA lesions.
DNA Pol ι acts sequentially with DNA Pol zeta, which is essential for damageinduced mutagenesis, to complete the DNA lesion bypass.
Therefore, replication involving DNA Pol ι is likely to be highly mutagenic.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human POLI protein.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa630-680 of Human POLI.
