Protein kinase C (PKC) family members influence a variety of cellular functions, including cell growth, cell differentiation, hormone secretion and membrane function.
PKC isoforms are calcium and phospholipid-dependent serine/threonine protein kinases.
Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC.
PKC delta phosphorylation on Thr 507 mediates inhibition of JAK2 and Stat3 function.
PKC delta phosphorylates and associates with Stat3 on Ser 727 following induction by IL-6 to negatively regulate the DNA binding and transcriptional activity of Stat3.
The Tyr 525, 523 and 565 residues in the catalytic domain are crucial for activation of PKC delta.
The Tyr 52, 155 and 187 residues of PKC delta reside within a regulatory domain.
The residue Ser 643 appears to be an autophosphorylation site.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human p-RKCD (S664) protein only when phosphorylated at Ser664. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to human PKCD around the phosphorylation site of Serine 664.
