Four proteins comprise the gamma-secretase complex: presenilin, nicastrin, aph-1, and PEN-2.
Together, these proteins mediate cell surface signaling pathways for a variety of type I membrane proteins, notably amyloid-beta precursor protein, a protein implicated in the development of Alzheimer's disease, via intramembrane proteolysis.
The proteins assemble into a proteolytically active complex in the golgi/trans-golgi network (TGN) compartments.
Assembly leads to autocleavage of presenilin into two subunits to create the active site of gamma-secretase, an important step in understanding the mechanisms involved in the etiology and possible treatment of Alzheimer's disease.
Applications:Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1:100Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in Tris, pH 7.4, 1% BSA, 0.05% sodium azide, 40% glycerol
Specificity：Recognizes endogenous levels of human PEN2 and does not cross-react with related proteins. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：FC IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human PEN2 at C-terminal.
