Parkin is a zinc-finger protein that is related to ubiquitin at the amino terminus.
The wild type Parkin gene, which maps to human chromosome 6q25.2- 27, encodes a 465aa full-length protein that is expressed as multiple isoforms ranging from 50-58kD.
Mutations in the Parkin gene are responsible for utosomal recessive juvenile Parkinson’s disease and commonly involve deletions of exons 3-5.
In humans, Parkin is expressed in a subset of cells of the basal ganglia, midbrain, cerebellum and cerebral cortex, and is subject to alternative splicing in different tissues.
Parkin expression is also high in the brainstem of mice, with the majority of immunopositive cells being neurons.
The Parkin gene has been identified in a diverse group of organisms including mammals, birds, frog and fruit flies, suggesting that analogous functional roles of the Parkin protein may have been highly conserved during the course of evolution.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human Parkin protein only when phosphorylated at Ser131. Species Crossreactivity: human
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from human Parkin around the phosphorylation site of S131.
