The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis.
A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases.
Nek5 (NimA-related protein kinase 5) is a 708aa protein that is related to NIMA, a protein that was originally discovered in Aspergillus nidulans and is necessary for entry into mitosis.
One of several members of the Ser/Thr protein kinase super family, Nek5 contains one protein kinase domain through which it catalyzes the ATP-dependent phosphorylation of target proteins.
Like NIMA, Nek5 may be involved in mitotic regulation and cell cycle control.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human NEK5 protein.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa150-200 of human NEK5.
