84-5132-73　［取扱停止］NDFIP1 50ul　505911

特徴

• Nedd4 family interacting protein-1 (Ndfip1), also referred to as N4WBP5, is a member of a family of highly conserved proteins.
• It has three transmembrane domains in its carboxy terminal and two PY motifs in its amino terminal.
• Ndfip1 is a 221aa, Golgi-associated protein that may play a role in Golgi structure and function.
• Ndfip1 binds the WW domains of a number of Nedd4 family members called HECT-type E3 ubiquitin ligases.
• Ndfip1 is strongly expressed in surviving neurons around a site of injury.
• This suggests that ubiquitination may be a possible survival strategy and Ndfip1 may act as a neuroprotective protein.
• Ndfip1 may also have an effect on the function of Itch, another E3 ubiquitin ligase.
• Expression of Ndfip1 and its association with Itch may be promoted by T cell activation.
• Mice lacking Ndfip1 exhibit inactivation of Itch and accumulation of JunB causing them to die prematurely with severe skin and lung inflammation.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.