Syntrophin is an adapter protein that functions to bind certain signaling molecules to the dystrophin-associated protein complex.
This complex connects the extracellular matrix to the intracellular cytoskeleton for construction and maintenance of the postsynaptic structures in the neuromuscular junction and the central nervous system.
Microtubule-associated serine/threonine-protein kinase 2 (MAST205) is a testis-specific, cytoplasmic protein that functions in a multi-protein complex in the maturation of spermatids.
MAST205 is involved in linking the dystrophin/utrophin network with microtubule filaments via Syntrophin.
By forming a complex with TRAF6, MAST205 regulates lipopolysaccharide-induced IL-12 synthesis in macrophages.
This leads to the inhibition of TRAF6 NF-˚B activation.
Two isoforms exist for MAST205 due to alternative splicing.
Isoform 1 represents the full length protein, while isoform 2 lacks the residues 327-396 and 1091-1113.
The N-terminus of MAST205 must be phosphorylated in order for ubiquitination to occur at the same site.
This ubiquitination leads to the degradation of MAST205 via proteasome-mediated proteolysis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human MAST2 protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa1205-1249 of human MAST2.
