Microtubule affinity-regulating kinase 2 (MARK2), also known as EMK1 (ELKL motif kinase 1) or Par1b, is a 788aa protein that is a member of the protein kinase superfamily, MARK subfamily.
Highly expressed in heart, brain, skeletal muscle and pancreas, MARK2 is essential for the asymmetric development of membrane domains around polarized epithelial cells.
Activation of MARK2 by phosphorylation on Thr 208 allows the protein to modulate the building of a columnar versus a hepatic epithelial cell.
MARK2 contains one KA1 (kinase-associated) domain, one protein kinase domain and one UBA domain.
MARK2 is expressed as 14 isoforms produced by alternative splicing events.
Some of these isoforms may function in graft rejection.
Applications:Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1:50-1:100Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in Tris, pH 7.4, 1% BSA, 0.05% sodium azide, 40% glycerol
Specificity：Recognizes endogenous levels of human MARK2 and does not cross-react with related proteins. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：FC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human MARK2 at C-terminal.
