The MAL-SRF interaction displays the predicted properties of a Rho-regulated SRF cofactor.
MAL is predominantly cytoplasmic in serum-starved cells, but accumulates in the nucleus following serum stimulation.
Activation of the Rho-actin signaling pathway is necessary and sufficient to promote MAL nuclear accumulation.
MAL N-terminal sequences, including two RPEL motifs, are required for the response to signaling, while other regions mediate its nuclear export (or cytoplasmic retention) and nuclear import.
MAL associates with unpolymerized actin through its RPEL motifs.
Constitutively cytoplasmic MAL derivatives interfere with MAL redistribution and Rho-actin signaling to SRF.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes levels of human MAL protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human MAL
