Protein transport across the nucleus is a selective, multi-step process involving several cytoplasmic factors.
Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion.
Two cytosolic factors centrally involved in the recognition and docking process are the karyopherin a1 and karyopherin beta1 subunits.
Karyopherin a1 functions in the recognition and targeting of substrates destined for nuclear import, while karyopherin beta1 serves as an adapter, tethering the karyopherin alpha1/substrate complex to docking proteins on the nuclear envelope termed nucleoporins.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunoprecipitation: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human KPNA2. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IF IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to full length human KPNA2.
