The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases.
Proteins belonging to this family function to catalyze the aminoacylation of tRNAs by their corresponding aa’s, thus linking aa’s with tRNA-contained nucleotide triplets.
IARS2 (Isoleucyl-tRNA synthetase), also known as IleRS (Isoleucine-tRNA ligase), is a 1012aa protein that is localized within the mitochondrial matrix.
Specifically belonging to the class I mitochondrial aminoacyl-tRNA synthetase family, IARS2 contains a characteristic Rossman fold, which is found in proteins that typically bind nucleotides.
The gene encoding IARS2 maps to human chromosome one, which is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human IARS2 protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa75-119 of human IARS2.
