Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.
This structure consists of approximately 146bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4).
The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
This gene is intronless and encodes a member of the histone H4 family.
Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
This gene is found in a histone cluster on chromosome 1.
This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry, CHIP.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:200-1:500Western Blot: 1:1000-1:3000Immunohistochemistry: 1:200-1:500Immunoprecipitation: 1:20-1:50CHIP: 1:20-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes endogenous levels of Histone H4 protein only when acetylated at K8. Species Crossreactivity: mouse, rat, Other (Wide Range)
Isotype：IgG
Calc Applications Abbrev：ChIP IF IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human Acetyl-Histone H4-K8.
