Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.
Nucleosomes consist of approximately 146bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4).
The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family.
Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry, CHIP, CHIP seq.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunoprecipitation: 1:50-1:200CHIP: 1:20-1:50CHIP Seq: 1:20-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 50% glycerol
Specificity：endogenous levels of human Histone H3 protein only when Di-methylated at Lys36. Species Crossreactivity: mouse, rat, other (Wide Range)
Isotype：IgG
Calc Applications Abbrev：ChIP IF IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic methylated peptide corresponding to residues surrounding K36 of human histone H3.
