HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1.
Human HDAC5 is composed of 1122aa residues.
The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule.
The N-terminal non-deacetylase domain does not show any significant homology with any published sequence.Both domains are required for HDAC5-mediated repression of gene transcription.
HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins.
The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human HDAC5 protein only when phosphorylated at Ser498. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding S498 of human HDAC5.
