FRS2 (also designated SNT or p90) is a lipid-anchored docking protein that becomes tyrosine phosphorylated in response to FGF or NGF stimulation and subsequently binds to GRB2/Sos complexes.
The GRB2 adapter protein links receptor tyrosine kinases to the Ras/MAPK signaling pathway but does not interact directly with FGF receptors.
FRS2 thus provides a link between activation of FGF and NGF receptors and the Ras/MAPK pathway.
FRS2 contains four Grb2 binding sites, a myristylation sequence and a PTP domain.
Myristylation of FRS2 is essential for membrane localization, tyrosine phosphorylation, GRB2/Sos recruitment and MAPK activation .
The function of FRS2 in FGF receptor signaling is analogous to that of IRS1 in response to insulin receptor stimulation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human FRS2 protein only when phosphorylated at Tyr436. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to human FRS2 around the phosphorylation site of Tyrosine 436.
