The estrogen receptor-alpha (ER) is a broadly expressed transcription factor which controls a number of genes involved in cellular differentiation and proliferation.
ER contains two transcriptionally active domains: the N-terminal domain (AF1) is constitutively active, while the C-terminal domain (AF-2) is hormone-dependent.
ER is subject to post-translational modification, including phosphorylation of serine residues 104, 106, 118, and 167.
This antibody specifically recognizes human estrogen receptor-alpha when phosphorylated at serine 167, a site which influences AF-1 dependent transcriptional activity.
This phosphorylation is catalyzed by the MAPK pathway, potentially through the 90kD ribosomal S6 kinase (pp90rsk1).
Serine 167 is also a consensus Akt substrate.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of ER-a protein. Species Crossreactivity: mouse and rat
