HRI (heme-regulated inhibitor kinase) phosphorylates the alpha subunit of eIF2alpha kinase, which plays an important role in translational regulation during heme deficiency.
HRI is activated in response to a number of environmental conditions, including heme deficiency, heat shock, and oxidative stress.
Autophosphorylation is essential for the activation of HRI, which causes an arrest of initiation of protein synthesis.
Both HSP 90 and HSC 70 are necessary for all stress-induced HRI activation.
Furthermore, HSC 70 is required for the folding and transformation of HRI into an active kinase and is subsequently required to negatively attenuate the activation of transformed HRI.
Both the N-terminus and the kinase insertion domain, which are unique to HRI, are involved in the heme binding and the heme regulation of HRI.
The human HRI gene maps to chromosome 7p22.1 and encodes a 630aa protein expressed mainly in erythroid cells.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human EIF2AK1 protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa580-630 of human EIF2AK1.
