84-5130-18　［取扱停止］EIF1AY 100ul　505728

特徴

• Ribosomes, made of Ribonucleoprotein, are the cellular machinery used to catalyze assembly of amino acids into polypeptide chains translated from a template messenger RNA sequence.
• In eukaryotes, the ribosome consists of a 40S (small) and a 60S (large) subunit.
• Protein synthesis initiates through the binding of mRNA to the 40S subunit followed by an aminoacylated tRNA and the 60S subunit.
• The initiating charged tRNA, which base pairs with an AUG start codon on the mRNA, is bound to the essential amino acid methionine (Met).
• eIF1AY (eukaryotic translation initiation factor 1A, Y-chromosomal), also known as eIF-1A Y isoform and eIF-4C, is a 144aa protein that is ubiquitously expressed and is thought to enhance protein biosynthesis.
• Ribosomal subunit dissociation and binding of the 40S subunit to MettRNA is assisted by eIF1AY.
• eIF1AY, a cytoplasmic protein, is encoded by a gene in the nonrecombining region of the Y chromosome (NRY) in human and on chromosome 18 in mouse and rat where it is known as eIF1A.
• eIF-1AX, a related protein known as eIF1AY in mouse, is a 144aa protein encoded by the X chromosome in human, mouse, and rat.
• eIF1AY and eIF1AX contain a single S1-like domain.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.