The DOCK2 gene encodes dedicator of cytokinesis 2 (DOCK 2), a hematopoietic cell-specific CDM family protein that is indispensable for lymphocyte chemotaxis.
DOCK 2 participates in the cytoskeletal rearrangements that are required for lymphocyte migration in response of chemokines.
This peripheral membrane protein activates Rac 1 and Rac 2 small GTPases, while presumably acting as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP.
DOCK 2 may also participate in IL-2 transcriptional activation through the activation of Rac 2.
DOCK 2 contains one DHR-1 (CZH-1) domain, one DHR-2 (CZH-2) domain and one SH3 domain.
The DHR-2 domain is a putative GEF activity mediator.
The DOCK 2 protein also co-localizes with F-Actin, and demonstrates expression in several human tissues, with the highest levels observed in peripheral blood leukocytes, thymus, spleen and liver.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human DOCK2 protein. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to human DOCK2.
