84-5129-99　［取扱停止］DICER1 100ul　505715

特徴

• The mammalian Dicer is a type III RNase-related protein with orthologs in yeast, Drosophila and Arabidopsis.
• Dicer contains an RNA-helicase motif, including a DEXH box in its amino-terminus and an RNase motif in the carboxyterminus.
• The gene encoding human Dicer maps to chromosome 14q32.13.
• Dicer is expressed in brain, heart, liver, lung, pancreas, kidney and placenta, and functions in the RNA interference pathway.
• Dicer cleaves short hairpin RNA precursors of approximately 70 bp into 21-23 bp dsRNAs that selectively target the destruction of homologous RNAs.
• Dicer localizes to the cytoplasm of mammalian cells.
• Specifically, it co-localizes with calreticulin in the endoplasmic reticulum.
• Although the cleavage of RNA by Dicer is ATP-independent, the product release necessary for the rapid turnover of this enzyme may be attributed to ATP.
• Immunoprecipitation studies indicate Dicer forms a complex with the PIWI domain of eIF2C translation initiation factors.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.