DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly.
Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division.
DDX54 (DEAD polypeptide 54), also known as DP97, is an 881aa protein that contains 2 bipartite nuclear localization signals, 3 nuclear receptor boxes (LXXLL motifs), a potential CoRNR box, and several stretches of glutamate and lysine residues.
DDX54 is ubiquitously expressed, with highest expression in pancreas and lung.
DDX54 co-localizes with ERalpha to structures in the nucleoplasm.
DDX54 represses ERalpha transcriptional activity and acts as a nuclear receptor co-repressor against ERbeta, progesterone, glucocorticoid and RARalpha.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human DDX54 protein.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa602-646 of human DDX54.
