84-5129-91　［取扱停止］DDX20 100ul　505709

特徴

• Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of motor neurons in the spinal cord.
• SMA is caused by deletion or loss-of-function mutations in the SMN (survival of motor neuron) gene.
• Gemin3, also known as DP103, DDX20, DEAD-box protein DP130 and DEAD/H box 20, is a protein product of human chromosome 1p13.2.
• It associates directly with SMN and is a part of the SMN complex containing Gemin2, Gemin4, Gemin5 and Gemin6, as well as several spliceosomal snRNP proteins.
• The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing of the nucleus.
• It is found in both the cytoplasm and the nucleus.
• The nuclear form is concentrated in subnuclear bodies called gems (for Gemini of the coiled bodies).
• Gemin3 also interacts with SmB, SmD2 and SmD3.
• It contains the conserved motif Asp-Glu-Ala-Asp (DEAD) characteristic of DEAD-box proteins.
• Gemin3 is a putative RNA helicase and shows ATPase activity.
• It is expressed in B and T cell neuroblastoma-derived cell lines, malignant melanoma tumor, normal testis and is expressed in low levels in colon, skeletal muscle, liver, kidney and lung.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.