Apoptosis, or programmed cell death, occurs duringnormal cellular differentiation and development of multicellular organisms.
Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas.
Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED3 protease family.
A novel DD containing molecule was recently cloned from mouse, human and monkey and designated Daxx.
Daxx is a death domain containing important intermediate in the Fas mediated apoptosis.
Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N terminal kinase (JNK) pathway.
It is widely expressed in fetal and adult human and mouse tissue, indicating its important function in Fas signaling pathways.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes endogenous levels of human Daxx protein only when phosphorylated at Ser668.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from human Daxx around the phosphorylation site of Serine 668.
