Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle.
These proteins have been well conserved through evolution and play a critical role in regulation of cell division.
Cyclin E, along with the three Cyclin D proteins and Cyclin C, has been shown to represent a putative G1 Cyclin on the basis of its Cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary.
Cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity：Recognizes human Cyclin E1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa360-400 of human Cyclin E1.
