CNOT7 (CCR4-NOT transcription complex, subunit 7), also known as CAF1 (CCR4-associated factor 1), hCAF-1 or BTG1-binding factor 1, is a member of the CAF1 family.
Localizing to the nucleus, CNOT7 is ubiquitously expressed and is believed to function as a transcription factor, playing a role in a wide variety of processes.
CNOT7 functions as a component of the evolutionarily conserved CCR4-NOT complex, a multi-subunit complex that participates in transcription as well as mRNA degradation.
CNOT7 and other subunits of the CCR4-NOT complex play a role in the regulation of nuclear hormone receptor activities.
CNOT7 directly binds to and interacts with RXR∫, TOB1, TOB2, BTG1, BTG2 and BTG3.
In addition, CNOT7 knockout mice are sterile and show an increase in bone mass, suggesting an important role for CNOT7 in spermatogenesis and as a suppressor of bone mass and BMP (bone morphogenetic protein) actions in osteoblasts.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human CNOT7 protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa10-54 of human CNOT7.
