The DNA fragmentation factor (DFF) is involved in the caspase-3 apoptotic pathway.
DFF is composed of two subunits, a 45kD subunit called DFF-45 (also designated ICAD, for inhibitor of CAD), and CPAN (caspase-activated nuclease), also designated CAD (caspase-activated deoxyribonuclease).
CPAN is a DNase that is responsible for DNA degradation during apoptosis.
CPAN is inhibited by DFF-45.
Caspase-3 acts to dissociate CPAN from DFF-45, allowing CPAN to enter the nucleus and degrade DNA.
CIDE-A and CIDE-B have been identified as proteins that share homology with the N-terminal region of DFF-45.
Like CPAN, CIDE-A and CIDE-B promote cell death and DNA fragmentation and are inhibited by DFF-45.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human CIDEB protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa104-148 of human CIDEB.
