84-5129-27　［取扱停止］Chk1, phosphorylated (S345) 100ul　505666

特徴

• Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins.
• Chk1 and Chk2 are involved in these processes as regulators of Cdks.
• Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage.
• Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis.
• Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues.
• It has also been shown that Chk1 can phosphorylate Wee 1 in vitro, providing evidence that the hyperphosphorylated form of Wee 1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.
• Chk1 is phosphorylated on Serine 345 (S345) in response to UV, IR and hydroxyurea (HU).
• Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development and tumor suppression.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.