Drosophila Flamingo is a seven pass transmembrane cadherin that is necessary for dendritic patterning and axon guidance.
Flamingo mammalian homologs play similar roles in neuronal development, during which they play an important role in cell-cell signaling.
Cadherin EGF LAG seven pass G-type receptors (CELSRs) are multi-pass membrane proteins that belong to the G protein-coupled receptor family of proteins.
Silencing CELSR2 gene expression results in signficant simplification of dendritic arbors of cortical pyramidal neurons and Purkinje neurons, which may be due to branch retraction.
In mouse, CELSR1, CELSR2 and CELSR3 are expressed in the nervous system at early developmental stages, and show expression patterns in the developing CNS.
CELSR2 is distributed at intercellular boundaries in the whisker and on processes of neuronal cells such as hippocampal pyramidal cells, Purkinje cells and olfactory neurons.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human CELSR3 protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human CELSR3.
