CDK5RAP3 (cyclin dependent kinase 5 regulatory subunit-associated protein 3), also designated C53 or IC53, may be involved in neuronal differentiation, cell proliferation and DNA repair.
The role CDK5RAP3 plays in neuronal differentiation may be attributed to its interaction with the Cdk5 activator protein called p35.
p35 physically associates with Cdk5 to activate enzymatic activity.
Cdk5 activity increases significantly during neuronal differentiation.
Upon transfection, CDK5RAP3 is capable of increasing the rate of cell proliferation, suggesting that it may play a role in tumorigenesis.
CDK5RAP3 is a regulatory component of the G2/M DNA damage checkpoint in response to genotoxic stress.
CDK5RAP3 is expressed in brain, heart, placenta, liver, skeletal muscle, lung, kidney and pancreas and is overexpressed in tumor tissue.
Three named isoforms exist for CDK5RAP3 as a result of alternative splicing events.
CDK5RAP3 contains two leucine zipper motifs, putative phosphorylation and potential N-myristoylation sites.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human CDK5RAP3 protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa211-255 of human CDK5RAP3.
