Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks).
Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression.
Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity.
Several Cdk proteins have been identified, including Cdk2–Cdk8, PCTAIRE-1–3, PITALRE and PITSLRE.
Cdk4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle.
This association with a D-type cyclin upregulates Cdk4 activity, whereas binding to the Cdk inhibitor p16 downregulates Cdk4 activity.
Activation of the Cdk4-cyclin complexes requires phosphorylation on a single threonyl residue of Cdk4, catalyzed by a Cdk-activating protein (CAK).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human CDK4 protein only when phosphorylated at Thr172. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding T172 of human CDK4.
