CD6 is a type I transmembrane glycoprotein that is present on mature thymocytes, peripheral T cells and a subset of B cells.
The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T-cell activation and the size difference between the CD6 forms is due in part to differences in phosphorylation state.
CD6 protein contains a 24aa signal sequence, 3 extracellular “scavenger receptor cysteine-rich” (SRCR) domains, a membrane-spanning domain, and a 44aa cytoplasmic domain.
CD6 shows significant homology to CD5.
CD6, which is also found in brain and B cell chronic lymphocytic leukemias, plays an important role in interactions of thymocytes with thymic epithelial cells.
CD6 molecules can physically associate with the TCR/ CD3 complex.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human CD6 protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa250-300 of human CD6.
