CD32 (also designated Fc gamma RII) is a low affinity receptor for the Fc fragment of aggregated IgG.
CD32 is responsible for the clearance of immunocomplexes by macrophages and also plays an important role in the regulation of antibody production by B cells.
IgG can noncooperatively bind either one or two highly glycosylated CD32 molecules, and this binding delivers a negative signal for B cells.
CD32 exists as several isoforms that are produced by alternative splicing of three distinct genes, A, B, and C.
These isoforms are designated Fc gamma RIIA, Fc gamma RIIB1, Fc gamma RIIB3, and Fc gamma RIIC.
All isoforms are present on monocytes, placental trophoblasts and endothelial cells.
In addition, the Fc gamma RIIB forms are present on B lymphocytes, and the Fc gamma RIIA and Fc gamma RIIC forms are found on neutrophils.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human CD32-B. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa250-300 of human CD32-B.
