84-5128-71　［取扱停止］CCDC88A 50ul　505628

特徴

• The actin-binding protein girdin (CCDC88A, GIV) is a non-receptor guanine nucleotide exchange factor (GEF) and part of a scaffold that mediates key signaling pathways during cell migration.
• Girdin protein structure includes an N-terminal Hook domain for microtubule interaction, a coiled-coil dimerization domain, a Galpha binding domain, a PI(4)P-binding domain, and a carboxy-terminal receptor-binding domain within a GEF motif.
• Akt kinase phosphorylates girdin at Ser1416, which promotes PI(4)P binding, localization of girdin to the membrane leading edge, and regulation of actin organization and cell motility.
• After growth factor receptor activation, girdin binds both G-protein and receptor to form an activation complex at the receptor cytoplasmic tail.
• The activation complex enhances receptor autophosphorylation and promotes downstream signaling that results in actin organization and cell migration.
• An activated growth factor phosphorylates girdin at its carboxy-terminal Tyr1764 and Tyr1798 residues to form an SH2 docking site for PI3K binding.
• The girdin GEF motif interacts with Galpha and leads to release of G beta, gamma, resulting in further PI3K activation and the completion of signal transduction from receptor to cytoskeleton.
• The cytoskeletal reorganization and cell migration properties of girdin are important in regulating several biological processes, including wound healing, angiogenesis, and cancer progression.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.