Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins.
It is synthesized as an inactive 43kD preprocathepsin D that is cleaved and glycosylated to form a 46kD procathepsin D and then further cleaved to produce 28kD and 15kD subunits (heavy and light chains, respectively).
Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway.
Numerous studies have suggested that cathepsin D plays a role in neuronal degradation and malignant transformation, particularily in breast cancer.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human CTSD protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa150-200 of human CTSD.
