Carbonic anhydrase IV (CA IV) is glycosylphosphotidylinositol-anchored to the outer surface of the plasma membrane where it catalyzes hydration-dehydration of CO2/HCO3 - .
CA IV is present on the plasma face of microcapillaries and in the choriocapillaris of the human eye.
CA IV facilitates renal acidification in the kidney and is responsible for the regulation of interstitial pH (pHo) transients in brain.
Impairment in targetting leads to disruption of HCO3 - secretion and associates with malfunction in cystic fibrosis cells.
Carbonic anhydrases are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide.
They participate in respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid.
Carbonic anhydrases show extensive diversity in tissue distribution and in their subcellular localization.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human CA4 protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa120-170 of human CA4.
