Apoptosis defines a set of cascades which, when initiated, programs the cell to undergo lethal changes such as membrane blebbing, mitochondrial breakdown and DNA fragmentation.
Bcl-2 is one of many key regulators of apoptosis which are essential for proper development, tissue homeostasis and protection against foreign pathogens.
BCLAF1 (BCL2-associated transcription factor 1), also known as BTF, is a 920aa protein that localizes to both the nucleus and the cytoplasm.
Expressed throughout the body, BCLAF1 functions as a death-promoting factor that interacts with and represses the transcription of Bcl-2, thereby influencing the regulation of apoptosis.
Overexpression of BCLAF1 results in the relocation of BCLAF1 to the nuclear envelope and the subsequent induction of apoptosis, an event that may occur as a result of DNA damage.
Four isoforms of BCLAF1 exist due to alternative splicing events.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human BCLAF1 protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa286-330 of human BCLAF1.
