Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents.
Control of autophagy was largely discovered in yeast and involves proteins encoded by a set of autophagy-related genes (Atg).
Formation of autophagic vesicles requires a pair of essential ubiquitin-like conjugation systems, Atg12-Atg5 and Atg8-phosphatidylethanolamine (Atg8-PE), which are widely conserved in eukaryotes.
Numerous mammalian counterparts to yeast Atg proteins have been described, including three Atg8 proteins (GATE-16, GABARAP, and LC3) and four Atg4 homologs (Atg4A/autophagin-2, Atg4B/autophagin-1, Atg4C/autophagin-3, and Atg4D/autophagin-4).
The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation.
Atg4 primes the Atg8 homolog for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7.
The Atg8 homolog is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate.
During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homolog.While Atg4B displays a broad specificity for Atg8 homologues, it preferentially cleaves LC3.
Mutation in the corresponding Atg4B gene can be associated with strong inhibition of autophagosome formation.
An excess of inactive Atg4B blocks lipidation of Atg8 homologues and inhibits autophagy.
This makes Atg4B a potential tool for characterization of the isolation membrane and other autophagy studies.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human ATG4B protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa75-119 of human ATG4B.
