The anaphase-promoting complex (APC) is composed of more than ten subunits, including APC1, APC2, APC4, APC5, APC7, APC8, APC10 and APC11.
The APC acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis.
APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division.
APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk).
APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2.
Specifically, CDC20 and CDH1 directly bind to and activate APC’s cyclin-ubiquitination activity.
In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC; thus preventing APC activation.
APC1, previously referred to as Tsg24 in mice, is the largest of the APC subunits and is encoded by a gene mapping to 2q12.1.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human ANAPC5 protein.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human ANAPC5.
