The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis.
alpha-protein kinase 1, also known as ALPK1, LAK or KIAA1527, is a 1244aa protein that contains one alpha-type protein kinase domain and belongs to the protein kinase superfamily.
Expressed at high levels in liver, alpha-protein kinase 1 recognizes and phosphorylates specific phosphorylation sites that are surrounded by peptides which have an å-helical conformation.
One of the targets of å-protein kinase 1 is Myosin I and, via its ability to phosphorylate Myosin I, alpha-protein kinase 1 is thought to play an important role in the apical trafficking of vesicles carrying raft-associated sucrase-isomaltase (SI).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human ALPK1 protein.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa11-55 of human ALPK1.
