5-lipoxygenase (5-LO) is expressed primarily in polymorphonuclear leukocytes, macrophages, and mast cells.
5-LO performs the first two catalytic reactions in the biosynthesis of leukotrienes, lipid metabolites that induce contractions of airway smooth muscle and increase vascular permeability during anaphylaxis.
The cellular localization of 5-LO varies between cell types.
In activated blood polymorphonuclear leukocytes 5-LO undergoes calcium dependent translocation from the cytosol to the nuclear envelope.
In alveolar macrophages, the majority of 5-LO is localized in the nucleus and, upon activation of these cells, intranuclear 5-LO binds to the nuclear membrane.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes endogenous levels of human ALOX5 protein only when phosphorylated at Ser271.Recognizes endogenous levels of human PDGFR-alpha protein only when phosphorylated at Tyr849.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding S271 of human ALOX5.
