Acrosin, a member of the peptidase S1 family, is a major protease present in the acrosome of mature mammalian spermatozoa.
Acrosin is a typical serine proteinase with trypsin-like cleavage specificity.
The zymogen form, proacrosin, is the precursor of Acrosin synthesized only in the postmeiotic stages of spermatogenesis.
The active enzyme functions in the lysis of the zona pellucida, allowing the penetration of sperm through the innermost glycoprotein layers of the ovum.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity:Recognizes endogenous levels of human ACR protein.
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to aa1-50 of human ACR.